Study of Withania somnifera with the spatial reference of phytochemical, FTIR and flavonoids quantification

Plant is oldest existing complete medical significance in the world. Its origins go back nearly 5000 years. In this research article make known on Withania somnifera (Ashwagandha) as Medicinal plants have therapeutic potential due to the presence of natural antioxidants functioning as reducing agents, free radical scavengers and quenchers of singlet oxygen. Majority of different medicinal plant and their antioxidant activity is due to bioactive compounds viz. flavones, isoflavones, flavonoids, anthocyanins, coumarins, lignans, catechins and isocatechins. Extraction of the bioactive plant constituents has always been a challenging task for the researchers. And this article also provides an overview of extraction, photochemical analysis, Flavonoide quantification and FTIR analysis of Withania somnifera (Ashwagandha). Key-Words: Medicinal Plants, Withania somnifera, Flavonoids Introduction It is evident that anti-oxidants play an important role in the prevention of cancer, cardiovascular and neurogenerative diseases. There has been a surge of research in its effect in animal models of atherosclerosis, hyperlipidemia, myocardial infarction, myocardial ischemia reperfusion injury, cerebral ischemia, cardiomyopathy, cardiac hypertrophy, cardiotoxicity and congestive heart failure. 13 different constituents of WS for their anti proliferative capabilities on lung, colon, central nervous system (CNS), and breast tumor lines (Jayaprakasam B et al 2003). Many pharmacological studies have been conducted to investigate the properties of ashwagandha and to authenticate its use as a multi-purpose medicinal agent. Studies on Withania somnifera suggests that it reduces tumor cell proliferation and enhances the effectiveness of radiation therapy while potentially mitigating undesirable side effects (Kaur et al., 2004). Withania somnifera Dunal (WS), commonly known as ashwagandha, has been used for centuries in Ayurvedic medicine to increase longevity and vitality (Altern Med Rev 2004). WS acts as an anti-inflammatory agent through inhibition of complement, lymphocyte proliferation, and delayed-type hypersensitivity (Rasool M et al 2006). * Corresponding Author E-Mail: [email protected] Scientific classification Kingdom: Plantae Order: Solanales Family: Solanaceae Genus: Withania Species: somnifera Fig .1: Withania somnifera Material and methods Plant material collection Plant withania somnifera ware collected from sanjivani ayurvedic nursery Bhopal during October month. Than dried up under the shed dry for three week furthermore crush it. Research Article [Nema et al., 3(3): Mar., 2012]